rabbit polyclonal antibodies against egf  (Danaher Inc)

Journal: International Journal of Molecular Sciences

Article Title: Searching for EGF Fragments Recreating the Outer Sphere of the Growth Factor Involved in Receptor Interactions

doi: 10.3390/ijms25031470

Figure Lengend Snippet: Sequences of each synthesized EGF fragment attached to the cellulose matrix. Division of peptides in terms of their ability to interact with polyclonal antibodies: very strong: ++; strong: +; moderate: +/−; no ability to interact: −. The staining intensities of antibody–EGF fragment complexes are also given.

Article Snippet: Rabbit polyclonal antibodies against EGF (Abcam 9695, 20 μL) were diluted 1:1000 in 1% BSA in PBS (20 mL, pH 7.4) and then incubated with cellulose sheets for 1 h at room temperature.

Techniques: Synthesized, Staining, Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Searching for EGF Fragments Recreating the Outer Sphere of the Growth Factor Involved in Receptor Interactions

doi: 10.3390/ijms25031470

Figure Lengend Snippet: Spot localization on membranes after SPOT synthesis of immobilized peptides and dot blot tests. The numbers 1–20 and letters A, B, and C represent 44 decapeptides of EGF attached to cellulose membranes, probed with a polyclonal antibody to EGF. Each individual spot (dot), which is a single peptide, has its own characteristic index. The peptide sequences are listed in . For example, the peptide in spot B1 has the amino acid sequence MYIEALDKYA.

Article Snippet: Rabbit polyclonal antibodies against EGF (Abcam 9695, 20 μL) were diluted 1:1000 in 1% BSA in PBS (20 mL, pH 7.4) and then incubated with cellulose sheets for 1 h at room temperature.

Techniques: Dot Blot, Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Searching for EGF Fragments Recreating the Outer Sphere of the Growth Factor Involved in Receptor Interactions

doi: 10.3390/ijms25031470

Figure Lengend Snippet: Fragments of EGF responsible for interacting with proteins: ( A ) fragments capable of interacting with specific polyclonal antibodies (fragments found by us), ( B ) fragments involved in binding with EGFR (fragments found by Ogiso et al. ). Amino acid residues marked in color are key for interactions.

Article Snippet: Rabbit polyclonal antibodies against EGF (Abcam 9695, 20 μL) were diluted 1:1000 in 1% BSA in PBS (20 mL, pH 7.4) and then incubated with cellulose sheets for 1 h at room temperature.

Techniques: Binding Assay

Journal: Scientific Reports

Article Title: A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection

doi: 10.1038/s41598-022-23824-3

Figure Lengend Snippet: HCV-JFH1-tau Lot B1 having M706L mutation can infect non-hepatic iPS cells via the CLDN6-dependent pathway. ( a ) Cellular expression patterns of various HCV entry factors. Huh7.5.1-8 and 253G1 cells were lysed, and equal protein amounts of each cell lysate (10 μg) were subjected to immunoblotting for the SRBI, EGFR, LDLR, CLDN1, CLDN6, OCLN, CD81, and GAPDH proteins. ( b ) Huh7.5.1-8 (squares), OKH-4 (triangles), and 253G1 (diamonds) cells were infected with HCV-JFH1-tau or HCV-JFH1-tau Lot B1 at 1.0 × 10 5 Geq/cell. At 0.5, 1, 1.5, and 2 days p.i., cellular HCV RNA contents were measured by qRT-PCR. Values are expressed as the ratio of OKH-4 at each day p.i. Data are presented as the mean ± S.D. (n = 6). *, p < 0.01 (vs. values of OKH-4 cells at each time point; Student’s t test). ( c ) 253G1 cells and Huh7.5.1-8 cells were infected with HCV-JFH1-tau Lot B1 at 1.0 × 10 5 Geq/cell in the presence of DMSO (white) or 1 μM sofosbuvir, a direct-acting antiviral agent (DAA, black). At 2 days p.i., HCV RNA contents in cells and culture supernatants were measured by qRT-PCR. Data are presented as the mean ± S.D. (n = 10 in 253G1 and n = 6 in Huh7.5.1-8). *, p < 0.01 (vs. values of DMSO treated cells; Student’s t test). ( d ) 253G1 cells (white) and S7-A cells (black) were preincubated with 5.0 μg/ml of control mouse IgG, or 5.0 μg/ml of anti-CD81 mAb (clone JS-81), 5.0 μg/ml of anti-CLDN6 mAb (clone 342927), 3.0 μg/ml of control rat IgG, or 3.0 μg/ml of mAb against OCLN (clone 1–3) for 30 min at room temperature and then infected with HCV-JFH1-tau Lot B1 at 1.0 × 10 5 Geq/cell. At 4 days p.i., cellular HCV RNA contents were measured by qRT-PCR. Values are expressed as the percentage of control mouse or rat IgG. Data are presented as the mean ± S.D. (n = 6).

Article Snippet: Rabbit polyclonal antibodies against EGFR were purchased from Cell signaling (Massachusetts, USA).

Techniques: Mutagenesis, Expressing, Western Blot, Infection, Quantitative RT-PCR, Control